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目的:探讨survivin、cyclinD1在结直肠癌中的表达及其与临床病理特征的关系。方法:43例结直肠癌组织标本,应用实时荧光定量PCR对survivin、cyclinD1基因在结直肠癌组织及癌旁正常组织中的mRNA水平进行定量检测,同时应用蛋白免疫印记(Westernblot)检测其在结直肠癌组织及癌旁正常组织中蛋白水平的表达。结果:结直肠癌组织survivin、cyclinD1mRNA和蛋白水平的表达均较癌旁正常组织表达显著上调,其差异有统计学意义(P﹤0.05);survivin、cyclinD1mRNA和蛋白水平的表达与患者性别、年龄、肿瘤部位、肿块大小、浸润深度无显著相关性(P﹥0.05),而与TNM分期、淋巴结转移有显著相关(P﹤0.05)。结论:提示survivin、cyclinD1基因在结直肠癌的发生、发展过程及预后中可能具有重要作用,有望成为新的肿瘤标志物或预后因子。
Objective: To investigate the expression of survivin and cyclinD1 in colorectal cancer and its relationship with clinicopathological features. Methods: The mRNA of survivin and cyclinD1 in colorectal cancer tissues and adjacent normal tissues was quantitatively detected by real-time fluorescence quantitative PCR in 43 cases of colorectal cancer tissues. At the same time, Western blotting was used to detect the expression of survivin and cyclinD1 in colorectal cancer tissues. Expression of protein in rectal cancer tissues and adjacent normal tissues. Results: The expressions of survivin, cyclinD1 mRNA and protein in colorectal cancer tissues were significantly up-regulated compared with normal tissues (P <0.05). The expressions of survivin, cyclinD1 mRNA and protein were significantly correlated with the gender, age, There was no significant correlation between tumor size, tumor size, and depth of invasion (P> 0.05), but significantly correlated with TNM stage and lymph node metastasis (P <0.05). Conclusion: The results suggest that survivin and cyclinD1 may play important roles in the development, progression and prognosis of colorectal cancer, and may be a new tumor marker or prognostic factor.