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目的探索建立超快速液相色谱(UFLC)法测定复方丹参片中三七皂苷R1、人参皂苷Rg1及Rb1。方法采用SHIMADZU Shim-pack XR-ODSⅢ(2.0 mm×75 mm,1.6μm)色谱柱;以乙腈-水作为流动相进行梯度洗脱;体积流量为0.4 mL/min;检测波长为203 nm;进样体积为3μL。结果三七皂苷R1、人参皂苷Rg1及Rb1分别在0.025 7～0.257 0、0.1012～1.012 0、0.104 4～1.044 0μg与峰面积呈良好的线性关系,平均加样回收率分别为96.7%、98.1%、98.8%。结论本方法在15min内可以将三七皂苷R1、人参皂苷Rg1及Rb1有效分离,节省了大量人力和流动相的消耗,为中药的质量控制技术提供参考方法。
Objective To establish an ultra fast liquid chromatography (UFLC) method for the determination of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Fufang Danshen Tablets. Methods A SHIMADZU Shim-pack XR-ODSⅢ (2.0 mm × 75 mm, 1.6 μm) column was used. The mobile phase was eluted with acetonitrile-water. The volume flow rate was 0.4 mL / min. The detection wavelength was 203 nm. The volume is 3 μL. Results The results showed that there was a good linear relationship between notoginsenoside R1, ginsenoside Rg1 and Rb1 in the range of 0.025 7 ~ 0.257 0,0.1012 ~ 1.012 0,0.104 4 ~ 1.044 0μg and the peak area, with the average recoveries of 96.7% and 98.1% , 98.8%. Conclusion This method can effectively isolate notoginsenoside R1, ginsenoside Rg1 and Rb1 within 15 min, saving a lot of manpower and mobile phase consumption, and providing a reference method for quality control of traditional Chinese medicine.