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The plasmids pBR322 and RSF1010,extracted from E.coli HB802 and E.coliC600 respectively,were treated with restrictive endonuclease EcoRI andligated with T4 DNA Iigase.C600 strains were then transformed with thehybrid plasmid(pSMMl),and thus253transformants were obtained.Thefigures of gel electrophoresis and electromicrograph proved that pSMMlwas a pBR322:RSF1010 composite hybrid.In E.coli C600 pSMMl obtainedresistance against ampieilline(Ap),tetracycline(Tc)and streptomycin(Sm).The orientation of pBR322 and RSF1010 in hybrid pSMMl and the failureof introduction of this hybrid into P.putida AC10 were discussed.
The plasmids pBR322 and RSF1010, extracted from E. coli HB802 and E. coli C600 were treated with restrictive endonuclease EcoRI and ligated with T4 DNA Iigase. C600 strain then then transformed with thehybridized plasmid (pSMM1), and thus253transformants were obtained.Thefigures of gel electrophoresis and electromicrograph demonstrated that pSMM1 was a pBR322: RSF1010 composite hybrid. In E. coli C600 pSMM1 obtained for resistance against ampieilline (Ap), tetracycline (Tc) and streptomycin (Sm). The orientation of pBR322 and RSF1010 in hybrid pSMM1 and the failure of introduction of this hybrid into P.putida AC10 were discussed.