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目的:建立微流控芯片非接触电导检测法测定盐酸吗啉胍片中盐酸吗啉胍含量。方法:在高电场强度条件下利用微流控芯片上特殊的微通道实现组分快速、高效的分离,以非接触电导检测器进行检测;探讨了缓冲液种类和浓度、添加剂种类和浓度、分离电压和进样时间等因素对分离检测的影响。结果:优化选择2 mmo·lL-1HAc+2 mmo·lL-1NaAc(pH=4.5)为缓冲溶液,0.3 mmo·lL-1十二烷基硫酸钠为添加剂,分离电压为2.20 kV,进样时间为10 s,2 m in内可实现盐酸吗啉胍快速分离和检测。在优化条件下,盐酸吗啉胍的线性范围为20～200μg.mL-1(r=0.9999),检出限为2.0μg.mL-1(S/N=3),RSD为1.4%,加标回收率为98.3%～101.2%。结论:方法简便快速,重复性好,适用于盐酸吗啉胍的快速检测和质量控制。
OBJECTIVE: To establish a non-contact conductivity detection method for the determination of Moroxydine hydrochloride in morpholine guanidine hydrochloride tablets by microfluidic chip. Methods: The rapid and efficient separation of components was carried out by special microchannels on a microfluidic chip under the condition of high electric field intensity. The detection was performed by a non-contact conductivity detector. The types and concentrations of buffers, the types and concentrations of additives, Voltage and injection time and other factors on the impact of separation testing. Results: Optimal choice was 2 mmo · lL-1HAc + 2 mmo · lL-1NaAc (pH = 4.5) as buffer solution, 0.3 mmo · L-1 sodium lauryl sulfate as additive, separation voltage of 2.20 kV, For 10 s, 2 m in, morpholine guanidine hydrochloride can be rapidly separated and detected. Under the optimal conditions, the linear range of Moroxydine hydrochloride was 20 ~ 200μg.mL-1 (r = 0.9999) with the detection limit of 2.0μg.mL-1 (S / N = 3) The standard recovery was 98.3% ~ 101.2%. Conclusion: The method is simple, rapid and reproducible. It is suitable for the rapid detection and quality control of Moroxydine Hydrochloride.