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实验用74只近交克隆系Wistar大鼠.切取供体神经,经冷冻和加热处理后,半数神经用正常兔血清处理,另半数用抗Laminin抗血清处理,然后分别植入对照组和实验组鼠体内.术后不同时间的电镜观察和统计数据表明:对照组再生轴突几乎都走在基底膜管内侧(92％),而实验组走在基底膜管内侧的轴突数(48％)与走在基底膜管外侧的轴突数(52％)几乎相等;轴突总数15天时实验组约为照对组的50％,30天时38％.实验组内轴突的长出时间较对照组晚约3天左右;实验组内巨噬细胞的数量和滞留时间均多于和长于对照组.这些结果表明Laminin在神经再生过程中不仅促进轴突生长成熟,引导神经方向性生长,亦对维持正常的再生微环境起了重要作用.
Totally 74 inbred clones were cultured in Wistar rats.The donor nerves were excised and after freezing and heat treatment, half of the nerves were treated with normal rabbit serum and the other half were treated with anti-Laminin antiserum and then were implanted into the control and experimental groups At the same time, electron microscopy and statistical data at different time points showed that almost all axons in the control group walked to the inside of the basilar membrane (92%), while the number of axons in the basilar membrane in the experimental group (48% And almost the same number of axons (52%) walking outside the basement membrane tube; the total number of axons 15 days when the experimental group was about 50% of the control group, 38 days at 30 days .Experimental group axon growth time than the control Group about 3 days later; the number and retention time of macrophages in the experimental group were longer than those in the control group.These results indicated that Laminin not only promote the axon growth maturation, but also direct the nerve growth in the process of nerve regeneration, Maintaining a normal regeneration microenvironment played an important role.