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采用羟基自由基体系、超氧阴离子自由基体系、烷基自由基引发的亚油酸氧化体系和DPPH体系对江蓠(Gracilaria)、麒麟菜(Eucheuma)、马尾藻(Sargassum)、海带(Laminaria)4种膳食纤维清除自由基的活性进行研究。结果表明,在羟基自由基体系中,江蓠、麒麟菜、马尾藻、海带4种膳食纤维的IC50分别为5.2mg/mL、5.5mg/mL、4.4mg/mL和4.1mg/mL;在超氧阴离子自由基体系中,江蓠、麒麟菜膳食纤维的IC50分别为4.8mg/mL和5.3mg/mL,而马尾藻、海带膳食纤维的最高清除率分别为36%和42%;在烷基自由基引发的亚油酸氧化体系中,江蓠、麒麟菜膳食纤维的最高清除率分别为36%和26%,马尾藻、海带膳食纤维的IC50分别为4.1mg/mL和3.5mg/mL;在DPPH体系中,江蓠、麒麟菜、马尾藻膳食纤维的最高清除率分别为31%、11%和26%,海带膳食纤维的IC50为6.2mg/mL。在各体系中,与相应的专一性阳性参照物对比,证明这4种海藻膳食纤维对自由基有一定的清除作用。
The hydroxyl free radical system, superoxide anion free radical system, alkyl free radical-initiated linoleic acid oxidation system and DPPH system were used to study the inhibitory effects on the growth of Gracilaria, Eucheuma, Sargassum, Laminaria, Four kinds of dietary fiber free radical scavenging activity were studied. The results showed that the IC50 of the four kinds of dietary fiber were 5.2mg / mL, 5.5mg / mL, 4.4mg / mL and 4.1mg / mL respectively in the hydroxyl free radical system; Oxygen anion free radical system, the riverbed, Eucheuma dietary fiber IC50 were 4.8mg / mL and 5.3mg / mL, while the highest clearance rate of Sargassum, seaweed dietary fiber were 36% and 42%; alkyl In the free radical initiated linoleic acid oxidation system, the highest clearance rates of dietary fiber from the euphorbia sinensis and eucheumae were 36% and 26%, respectively. The IC50 of Sargassum and kelp dietary fiber were 4.1mg / mL and 3.5mg / mL, respectively. In the DPPH system, the highest clearance rates of dietary fiber from seaweed, Eucheuma and Sargassum were 31%, 11% and 26%, respectively. The IC50 of kelp dietary fiber was 6.2mg / mL. In each system, compared with the corresponding specific positive reference, it was proved that the four kinds of seaweed dietary fiber had certain scavenging effects on free radicals.