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The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate(AB023574),Streptococcus dysqalactiae isolate(HM146084.1,AB537922,EU075033),Streptococcus difficilis isolate(AB112407) accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs.
The tested strain from typical clinical cases, and the physio-chemical characteristics, 16 S rRNA gene sequence and drug sensitivity challenge property were analyzed. This isolate was determined as Streptococcus agalactiae, 16 S rRNA gene fragment of 1 280 bp in length. Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate (AB023574), Streptococcus dysqalactiae isolate (HM146084.1, AB537922, EU075033), Streptococcus difficilis isolate (AB112407) accessed in GenBank. Isolate was sensitive to ciprofloxacin , neomycin, spectinomycin, furadantin and enoxacin.The results lay foundation for the effective control, rational medicine and vaccine preparation for Streptococcus disease on bullfrogs.