国内在尘肺、免疫等研究中应用的巨噬细胞都从动物的肺或腹腔中分离。巨噬细胞在体外一般不再进行DNA复制,不能在体外传代,每次实验必须宰杀动物、分离细胞。最近,我们从英国医学研究委员会尘肺研究所引进P388D1细胞株。这种细胞株有吞噬功能,能在体外传代。目前,已在实验室中生长繁殖。为了进一步推广使用,现把它的特性、生长条件和培养方法等作一介绍。 Domestic use in pneumoconiosis, immune and other studies of macrophages are isolated from the animal’s lung or abdominal cavity. Macrophages are generally no longer in vitro DNA replication, can not pass in vitro, each experiment must slaughter animals, cells. Recently, we introduced the P388D1 cell line from the Institute of Pneumoconiosis at the British Medical Research Council. This cell line phagocytosis, in vitro passaging. Currently, it has been in the laboratory growth and reproduction. In order to further promote the use, now its characteristics, growth conditions and training methods for an introduction.