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目的 确定多发性骨髓瘤 (MM)骨髓活检标本中是否存在人类疱疹病毒 8型 (HHV8)感染 ,病毒负荷量的差异与临床表现之间的关系。方法 实时荧光定量PCR确定骨髓活检标本中的HHV8病毒的负荷量 ,采用筑巢式PCR扩增患者外周血、骨髓穿刺和骨髓活检标本中HHV8KS330 2 3 3Bam片段 ,用逆转录 PCR方法了解HHV8病毒的致病基因病毒源白细胞介素 6 (vIL 6 )和病毒源干扰素调节因子 1(vIRF 1)的表达情况。结果 多数MM患者的骨髓活检标本可检出HHV8,荧光定量PCR发现阳性率为 6 9 6 % (16 / 2 3) ;筑巢式PCR时的阳性率为 82 6 % (19/ 2 3) ,骨髓穿刺和外周血标本的阳性率分别为 4% (1/ 2 5 )和 0。临床分析表明病毒的检出可能与化疗有关。vIRF 1在骨髓活检标本中表达率很高 ,为 83 3% (10 / 12 ) ,vIL 6的表达率为 0。结论 多发性骨髓瘤和HHV8感染有关 ,高表达的vIRF 1在多发性骨髓瘤发病的过程中可能起一定的作用
Objective To determine the presence of human herpesvirus 8 (HHV8) infection in multiple myeloma (MM) bone marrow biopsy specimens and the relationship between viral load and clinical manifestations. Methods Real-time fluorescence quantitative PCR was used to determine the load of HHV8 virus in bone marrow biopsy specimens. Nested PCR was used to amplify HHV8KS330 2 3 3Bam fragments in peripheral blood, bone marrow biopsy and bone marrow biopsy samples. The HHV8 virus was identified by reverse transcription PCR. The pathogenic gene virus was derived from the expression of interleukin 6 (vIL 6) and viral interferon regulatory factor 1 (vIRF 1). Results HHV8 could be detected in bone marrow biopsy specimens of most patients with MM. The positive rate was 696.6% (16/23) by fluorescence quantitative PCR and 82.6% (19/23) in nested PCR. The positive rates of bone marrow biopsy and peripheral blood specimens were 4% (1/25) and 0, respectively. Clinical analysis shows that the detection of the virus may be related to chemotherapy. The expression rate of vIRF 1 in bone marrow biopsy specimens was high, 83 3% (10 / 12), and the expression rate of vIL 6 was 0. Conclusion Multiple myeloma is associated with HHV8 infection. Highly expressed vIRF 1 may play a role in the pathogenesis of multiple myeloma