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目的:观察参麦汤对糖耐量低减大鼠白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及肝组织病理变化的影响。方法:高脂高糖饲料喂养结合腹腔注射小剂量链脲佐菌素溶液的方法复制糖耐量低减大鼠模型;用参麦汤治疗;实验结束后采用放射免疫法测血清TNF-α、采用酶联免疫(ELISA)法测定IL-6、处死动物后取肝组织常规石蜡包埋切片,HE染色,光镜下观察肝组织形态的变化。结果:和正常组比较,模型组TNF-α、IL-6的浓度升高,有显著性差异(TNF-αP<0.05,IL-6 P<0.05);与模型组比较,各干预组均可降低TNF-α、IL-6的浓度,其中以参麦汤组为著(P<0.05),参麦汤加运动组(P<0.05)、运动组(P<0.05)亦有明显差异。组织形态观察显示:糖耐量异常大鼠的肝组织存在脂肪样变;各治疗组对糖耐量异常大鼠肝组织的作用,以参麦汤加运动组改善较理想。结论:糖耐量低减期存在肝的脂肪样变;参麦汤可改善糖耐量异常大鼠的IL-6、TNF-α水平,并在一定程度上逆转病变肝组织。
Objective: To observe the effects of Shenmai Tang on the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and pathological changes of liver in rats with impaired glucose tolerance. Methods: The model of impaired glucose tolerance was established by intraperitoneal injection of low-dose streptozotocin solution. The rats were treated with Shen Mai Tang. After the experiment, serum TNF-α was measured by radioimmunoassay IL-6 was detected by enzyme-linked immunosorbent assay (ELISA), and the paraffin-embedded sections of liver tissue were harvested and stained with hematoxylin-eosin (HE). The morphology of liver was observed under light microscope. Results: Compared with the normal group, the concentrations of TNF-α and IL-6 in the model group were significantly increased (TNF-αP <0.05, IL-6, P <0.05) (P <0.05), Shenmai Tonga Decoction group (P <0.05), and exercise group (P <0.05). Histological observation showed that there was fatty change in the liver tissue of rats with impaired glucose tolerance. The effect of each treatment group on liver tissue of rats with impaired glucose tolerance was better than that of Shenmai Tonga plus exercise group. CONCLUSIONS: Liver steatosis exists in the period of low glucose tolerance. Shenmai Decoction can improve the level of IL-6 and TNF-α in rats with impaired glucose tolerance and reverse the pathological changes of liver tissue to a certain extent.