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目的研究维生素C(Vitamin C,Vit.C)对大鼠背根神经节神经元轴突生长的影响,为探讨神经再生新策略提供基础。方法取新生SD大鼠背根神经节(dorsal root ganglion,DRG)进行神经元分离培养。在培养液中加入不同浓度的维生素C(0,100,200,400μmol/L)处理24 h后通过βIII tubulin,Rho A免疫组化以及鬼笔环肽染色对神经元突起的数量和长度、生长锥伪足数量、神经元胞体大小及其内Rho A表达强度进行分析。结果神经元突起的数量、长度,生长锥伪足的数量以及神经元胞体面积均随维生素C的浓度增高而增长,而神经元内Rho A表达强度则相应降低。结论维生素C可促进体外大鼠背根节神经元突起的生长,这可能与Rho A表达下调相关。
Objective To study the effects of Vitamin C (Vit.C) on the axonal growth of dorsal root ganglion neurons in rats and provide the basis for exploring new strategies of nerve regeneration. Methods Dorsal root ganglion (DRG) of neonatal SD rats were used for neuronal culture. The number and length of protuberances, the number of pyramidal neurons, the number of pyramidal neurons, the number of neurons, the number of protuberances, The size of neuronal soma and the expression of Rho A were analyzed. Results The number of neurons, the number of pyramidal neurons and the area of neurons were increased with the increase of vitamin C. The intensity of Rho A expression in neurons decreased accordingly. Conclusion Vitamin C can promote the growth of neurite outgrowth in rat dorsal root ganglion in vitro, which may be related to the down-regulation of Rho A expression.