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目的研究表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）及其受体（ＥＧＦｒｅｃｅｐｔｏｒ，ＥＧＦＲ）在食管鳞状细胞癌中的表达。方法用免疫组织化学ＡＢＣ法分析并研究染色结果与病理改变之间的关系。ＥＧＦ染色，以正常人颌下腺作为阳性对照。ＥＧＦＲ以正常人胎盘组织为阳性对照。ＥＧＦ和ＥＧＦＲ染色一抗的浓度分别为１２００，１１００，４℃孵育４８ｈ。结果ＥＧＦ强阳性１８例，弱阳性１６例，ＥＧＦＲ强阳性１８例，弱阳性１６例。ＥＧＦ和ＥＧＦＲ的表达分为两种类型：Ｍ型：癌组织本身免疫组织化学反应强弱不同，Ｄ型：癌组织中免疫组织化学反应强度均一。Ｄ型与Ｍ型在ＥＧＦ、ＥＧＦＲ的表达中较一致。Ｍ型较多发生淋巴结转移（Ｐ＜０．０５），并且强阳性组中Ｍ型所占比例较大。结论ＥＧＦ及ＥＧＦＲ强阳性表达同食管癌病人的预后有关。
Objective To investigate the expression of epidermal growth factor (EGF) and its receptor (EGF receptor, EGFR) in esophageal squamous cell carcinoma. Methods The relationship between staining results and pathological changes was analyzed by immunohistochemical ABC method. EGF staining was performed with normal human submandibular gland as a positive control. EGFR normal human placental tissue as a positive control. The concentrations of primary antibodies stained with EGF and EGFR were 1200, 1100 and incubated at 4°C for 48 h. Results EGF was strongly positive in 18 cases, weakly positive in 16 cases, strongly positive in EGFR in 18 cases, and weakly positive in 16 cases. The expression of EGF and EGFR is divided into two types: M-type: the immunohistochemical reaction of cancer tissue itself is different, D-type: the intensity of immunohistochemical reaction in cancer tissue is uniform. D-type and M-type were more consistent in the expression of EGF and EGFR. M-type lymph node metastasis occurred more frequently (P<0.05), and the proportion of M-type in the strong-positive group was larger. Conclusion The strong positive expression of EGF and EGFR is related to the prognosis of patients with esophageal cancer.