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目的 :探讨热休克蛋白 70 ( HSP70 )和糖调节蛋白 94( grp94)在人胃癌细胞BGC- 82 3中的表达及其意义。方法 :利用 En Vision免疫细胞化学、双抗体标记免疫荧光和流式细胞仪方法检测和分析人胃癌细胞 BGC- 82 3中 HSP70和grp94的表达及其与细胞周期的关系。结果 :胃癌细胞存在 HSP70和 grp94的高表达 ,免疫细胞化学显示 HSP70和 grp94主要定位于胞浆 ;免疫荧光法和流式细胞仪检测显示 HSP70的表达阳性率为 84.9% ,grp94为 79.7% ,与阴性对照组存在明显的差异 ;在整个细胞周期均存在 HSP70和 grp94的持续表达。结论 :胃癌细胞存在 HSP70和 grp94的高表达 ,在整个细胞周期 HSP70和 grp94均持续表达 ,其表达与细胞周期无明显关系。 HSP70和 grp94在人胃癌细胞中的表达特点为瘤苗的研究奠定了基础。
Objective: To investigate the expression and significance of heat shock protein 70 (HSP70) and glyco-regulatory protein 94 (grp94) in human gastric cancer cells BGC-823. METHODS: The expression of HSP70 and grp94 in human gastric cancer cells BGC-823 and its relationship with cell cycle were detected and analyzed by En Vision immunocytochemistry, double-antibody labeling immunofluorescence and flow cytometry. Results: The expression of HSP70 and grp94 in gastric cancer cells was high. Immunocytochemistry showed that HSP70 and grp94 were mainly localized in the cytoplasm. Immunofluorescence and flow cytometry showed that the positive rate of HSP70 expression was 84.9% and grp94 was 79.7%. There was a significant difference in the negative control group; there was continuous expression of HSP70 and grp94 throughout the cell cycle. Conclusion : There is high expression of HSP70 and grp94 in gastric cancer cells. HSP70 and grp94 are continuously expressed in the whole cell cycle, and their expression is not related to the cell cycle. The expression of HSP70 and grp94 in human gastric cancer cells laid the foundation for the study of tumor vaccines.