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目的:观察细胞间粘附分子-1(intercellular adhesionmolecule-1,ICAM-1)含量变化与角膜移植排斥反应的关系。方法:利用缝线诱发新西兰白兔角膜新生血管(corneal neovascularization,CNV)后,制作穿透性角膜移植(penetrating keratoplasty,PKP)模型4组,术后记录移植排斥指数(rejection index,RI);记录植片存活时间;应用酶联免疫吸附(ELISA)双抗体夹心法检测房水及外周血中sICAM-1的含量,免疫组织化学染色法观察ICAM-1的表达。结果:排斥组植片平均存活12.4±1.3d;正常房水和血清中均可检测到少量的sICAM-1,它们的浓度分别为(16.6±3.6)ng/L(,95.2±6.3)ng/L。术后排斥组房水及外周血中sICAM-1含量即升高,至排斥反应前达最高水平分别为(53.9±19.2)ng/L,(378.8±30.6)ng/L,在观察期内维持高水平,排斥反应发生时,免疫组化染色ICAM-1呈强阳性表达。结论:ICAM-1在角膜移植免疫排斥反应中发挥重要作用,术后外周血中sICAM-1浓度变化对排斥反应的发生有一定预测和早期诊断作用。
Objective: To observe the relationship between the content of intercellular adhesion molecule-1 (ICAM-1) and the rejection of corneal allografts. Methods: New Zealand white rabbits were induced by corneal neovascularization (CNV). Four groups of penetrating keratoplasty (PKP) models were established. The graft rejection index (RI) Survival time of sICAM-1 was detected by enzyme-linked immunosorbent assay (ELISA). The expression of sICAM-1 in aqueous humor and peripheral blood was detected by immunohistochemical staining. Results: In the rejection group, the mean survival time was 12.4 ± 1.3 days. A small amount of sICAM-1 was detected in both normal and aqueous humor, with concentrations of (16.6 ± 3.6) ng / L (95.2 ± 6.3) ng / L. The levels of sICAM-1 in aqueous and peripheral blood of patients with rejection after operation increased to (53.9 ± 19.2) ng / L and (378.8 ± 30.6) ng / L before rejection, respectively, and maintained within the observation period High-level, rejection reaction occurs, immunohistochemical staining ICAM-1 was strongly positive expression. CONCLUSION: ICAM-1 plays an important role in the immune rejection of corneal allografts. The change of sICAM-1 concentration in peripheral blood after the operation has some predictive and early diagnostic effects on the rejection.