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通过MNP对肌酸激酶的化学修饰,在变性条件下再用过量IAM修饰、胰酶水解、FPLC分离等一系列步骤,得到肌酸激酶的两个纯化的MNP修饰肽段,通过氨基酸组成分析证明MNP修饰在Cys-145及Cys-253位的两个内埋巯基上。
Two purified MNP modified peptides of creatine kinase were obtained by chemical modification of creatine kinase by MNP, excessive IAM modification under denaturing conditions, trypsin hydrolysis and FPLC separation. The amino acid composition analysis showed that MNP modified at both Cys-145 and Cys-253 buried thiol.