The current study evaluated the effects of oxalic acid (OA) application on the growth and Mn phytoremediation efficiency of Polygonum pubescens Blume cultivated in three different manganese(Mn)-contaminated soils sampled from an unexplored area (US),an explored area (ES) and a tailing area (TS) of the Ertang Mn mine,South China.The supplied levels of OA were 0 (control),1 (low level),3 (medium level),and 9 (high level) mmol/kg,referred to as CK,OA1,OA3 and OA9,respectively.The results revealed that the average water-extractable Mn concentrations US,ES and TS amended with OA increased by 214.13,363.77 and 266.85％,respectively.All OA supply levels increased plant growth and Mn concentrations in US.The low OA supply level increased plant growth in ES and TS;however,contrasting results were found for the medium and high OA supply levels.Plant Mn concentrations and total Mn increased in ES and TS in response to all OA supply levels.Total Mn in the aerial parts increased by 81.18,44.17 and 83.17％ in US,ES and TS,respectively;the corresponding percentages for the whole plants were 81.53,108.98 and 77.91％,respectively.The rate of ·O2-production and malondialdehyde (MDA) concentrations increased in response to OA amendment,especially the medium and high OA supply levels in ES and TS.In general,antioxidant enzymes might play a vital role in alleviating Mn stress in plants cultivated in US,while non-enzymatic antioxidants might be the main factor for plants cultivated in ES and TS.