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目的 探讨南京市人群中NQO1、CYP1A1、mEH exon3、mEH exon4基因多态性与肺癌易感性的关系。方法 用病例 -对照研究方法 ,收集南京市区原发性肺癌患者 84例 ,其中鳞癌 35例 ,腺癌 49例 ,同时选择对照 84例。采用PCR技术 ,对样本DNA进行NQO1、CYP1A1、mEH exon3、mEH exon4基因型的检测 ,并分析各基因型与肺癌易感性的关系。结果 南京市区人群NQO1、CYP1A1和mEH exon4与肺癌易感性没有明显关系。mEH exon3基因型与肺鳞癌发生有关 ,野生型个体可降低肺鳞癌发病的风险 (OR =0 .32 ,95 %CI:0 .0 0 78～ 0 .63) ,杂合型和突变型个体患肺鳞癌的危险性明显高于野生型个体 (OR=3 .1 ,95 %CI:0 .0 8～ 6 .1 2 ) ;考虑吸烟因素后 ,mEH exon3基因型与吸烟者肺癌发生有关 ,野生型个体可使肺癌发病风险性降低 (OR =0 .1 8,95 %CI:0 .0 6～ 0 .2 9) ,杂合型和突变型个体患肺癌的危险性增高 (OR =5 .66 ,95 %CI:2 .0 1～ 9.30 )。结论 南京市人群中NQO1、CYP1A1、mEH基因的分布情况与国内外的相关报道存在一定差异 ,种族差异、地域不同可能是造成上述基因分布不同的重要原因。南京市人群中mEH exon3基因杂合型和突变型与肺鳞癌发生有关 ,与吸烟者肺癌发生关系更为密切
Objective To investigate the relationship between polymorphisms of NQO1, CYP1A1, mEH exon3, mEH exon4 and lung cancer susceptibility in Nanjing population. Methods A case-control study was conducted in 84 patients with primary lung cancer in Nanjing, including 35 squamous cell carcinomas and 49 adenocarcinomas, and 84 patients were selected as control. The genotypes of NQO1, CYP1A1, mEH exon3 and mEH exon4 were detected by PCR, and the relationship between genotypes and lung cancer susceptibility was analyzed. Results There was no significant relationship between NQO1, CYP1A1 and mEH exon4 in Nanjing population and susceptibility to lung cancer. The mEH exon3 genotype was associated with the occurrence of squamous cell carcinoma of the lung, and the wild type individuals could reduce the risk of squamous cell lung cancer (OR = 0.32, 95% CI: 0.078-0.63), heterozygous and mutant The risk of lung squamous cell carcinoma was significantly higher in individuals than in wild-type individuals (OR = 3.1, 95% CI: 0.08-6.12). After considering smoking factors, the mEH exon3 genotype was associated with lung cancer in smokers Related, wild-type individuals can reduce the risk of lung cancer (OR = 0.18, 95% CI: 0.06 to 0.29), heterozygous and mutant individuals with increased risk of lung cancer (OR = 5 .66, 95% CI: 2.01 ~ 9.30). Conclusion The distribution of NQO1, CYP1A1 and mEH genes in Nanjing population is different from the reports at home and abroad. Racial differences and geographical differences may be the important reasons for the above-mentioned gene distribution differences. Nanjing population mEH exon3 gene heterozygous and mutant type associated with the occurrence of squamous cell carcinoma of the lung, lung cancer and smokers have a closer relationship