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为建立匍匐翦股颖愈伤组织再生体系,通过种子愈伤组织诱导和再生实验,筛选出克隆系Penn A4-1。进一步以克隆系的茎段为材料,建立了高效稳定的单一基因型Penn A4-1愈伤组织诱导和再生体系。愈伤组织诱导和愈伤组织增殖的最适培养基均为MS+1 mg/L 2,4-D,出愈率和增殖率分别达到91.67%和169.25%;愈伤组织分化的最适培养基为MS+2 mg/L 6-BA+0.1 mg/L NAA,分化率达到52.38%。高效愈伤组织再生体系的建立,为匍匐翦股颖品种Penn A4持续稳定的基因工程研究奠定了重要的基础。
In order to establish creeping bentgrass callus regeneration system, the clone Penn A4-1 was screened through seed callus induction and regeneration experiments. Further, the stem segments of clonal lines were used as materials to establish a highly efficient and stable single-genotype Penn A4-1 callus induction and regeneration system. The optimum culture medium for callus induction and callus proliferation was MS + 1 mg / L 2,4-D, and the callus formation rate and proliferation rate reached 91.67% and 169.25% respectively. The optimum culture of callus differentiation Based on MS + 2 mg / L 6-BA + 0.1 mg / L NAA, the differentiation rate reached 52.38%. The establishment of a highly efficient callus regeneration system laid an important foundation for the continuous and stable genetic engineering of Creeping creeping bentgrass Penn A4.