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目的探讨丙烯酰胺染毒对雄性小鼠海马组织结构和Caspase-3、GFAP表达的影响。方法健康雄性昆明种小鼠40只,随机分为正常对照组和低、中、高浓度3个剂量实验组,每组10只。对照组小鼠腹腔注射生理盐水;实验组小鼠分别代以5、30和60 mg/(kg.d)丙烯酰胺水溶液腹腔注射。在10d时分别处死各组小鼠,快速取出海马,Zamboni固定液固定,石蜡包埋,常规制片,苏木素-伊红(HE)染色,Caspase-3和GFAP免疫组织化学染色,光学显微镜观察。结果实验组小鼠海马同对照组相比显微结构未见明显改变。与对照组比较,各剂量组小鼠海马神经元Caspase-3阳性表达明显增强(P<0.01),但各剂量组间未见明显改变(P>0.05);各剂量组小鼠海马胶质细胞GFAP阳性表达明显增强(P<0.01),高剂量组表达较低、中剂量组增强(P<0.05)。结论丙烯酰胺染毒可造成小鼠海马神经元和神经胶质细胞损伤。
Objective To investigate the effect of acrylamide on the expression of Caspase-3 and GFAP in the hippocampus of male mice. Methods Forty healthy male Kunming mice were randomly divided into normal control group and low, medium and high concentration three dose experimental groups, 10 in each group. Control mice were injected intraperitoneally with normal saline. The mice in experimental group were injected intraperitoneally with 5, 30 and 60 mg / (kg.d) acrylamide solution respectively. The mice in each group were sacrificed on the 10th day, and the hippocampus was removed quickly. The Zamboni fixatives were fixed, paraffin-embedded, routinely prepared, HE staining, Caspase-3 and GFAP immunohistochemical staining and light microscopy. Results There was no significant change in the microstructure of hippocampus in the experimental group compared with the control group. Compared with the control group, Caspase-3 positive expression of hippocampal neurons in each dose group was significantly increased (P <0.01), but there was no significant difference between the various dose groups (P> 0.05); The hippocampal glial cells GFAP positive expression was significantly increased (P <0.01), high-dose group was lower, middle dose group increased (P <0.05). Conclusion Acrylamide exposure can cause damage of hippocampal neurons and glial cells in mice.