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目的:探讨将浆膜腔积液制作细胞块并进行免疫组化染色进一步诊断的方法。方法:对84例浆膜腔积液标本分别制作细胞涂片、细胞块,并对细胞块切片进行免疫组化染色。结果:细胞块行免疫组化染色效果明显优于传统离心涂片。结论:浆膜腔积液细胞块的应用不仅能提高细胞学诊断的阳性率及准确率,而且细胞蜡块能长期保存并反复切片,进行后续各种检查,包括免疫组化、特殊染色及FISH及PCR等多种分子病理检查,拓展了体液细胞学的应用价值
OBJECTIVE: To explore the method of making the cell mass by serous effusion and further diagnosing it by immunohistochemistry. Methods: 84 cases of serous effusion samples were made of cell smears, cell blocks, and cell mass sections for immunohistochemical staining. Results: Immunohistochemical staining of cell masses was significantly better than traditional centrifugal smears. Conclusion: The application of serous effusion cell mass can not only improve the positive rate and accuracy of cytological diagnosis, but also preserve long-term paraffin sections of paraffin-embedded cells and follow-up examinations including immunohistochemistry, special staining and FISH And PCR and other molecular pathological examination, expanding the application of humoral cytology