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从100个随机引物中筛选出28个多态性稳定的引物,对34个桂花品种、6个桂花野生居群个体、5个木犀属其他种进行了遗传多样性和亲缘关系的RAPD分析。共检测了231个位点,其中209个位点为多态位点(90.4％)。不同品种群的多态性位点频率差异明显,按大小依次排序为:银桂品种群>四季桂品种群、金桂品种群>丹桂品种群。聚类结果与形态分类结果不完全一致。利用来自引物A11和M18扩增的21条多态性条带构建了桂花品种的DNA指纹图谱,该图谱可以方便地应用于桂花品种的鉴定。不同桂花品种之间存在明显差异。研究结果表明,RAPD技术可用于桂花品种的鉴定和亲缘关系的探讨。
A total of 28 primers were selected from 100 random primers. Genetic diversity and genetic relationship among 34 osmanthus species, 6 wild populations of Osmanthus fragrans and 5 species of Osmanthus were analyzed by RAPD. A total of 231 loci were detected, of which 209 loci were polymorphic (90.4%). The frequency of polymorphic loci in different breeds was significantly different, sorted by size in turn as follows: Silver Gui-breed> Siji Gui-breed, Jing-Gui-breed> Dan-Gui-breed. The clustering results are not completely consistent with the morphological classification results. The DNA fingerprinting of sweet-scented osmanthus species was constructed by using 21 polymorphic bands amplified from primers A11 and M18, which can be easily applied to the identification of sweet-scented osmanthus varieties. There are obvious differences between different sweet-scented osmanthus cultivars. The results show that RAPD technology can be used to identify Osmanthus varieties and genetic relationship.