Background: Synthetic biology has attracted enormous attention in recent years.A key focus of synthetic biology is to utilize modular biological building blocks to assemble the cell-based circuits.Results: Scientists have programmed the living organisms using these circuits to attain multiple,delicate and welldefined functions.With the integration of tools or technologies from other disciplines,these rewired cells can achieve even more complex tasks.Conclusions: In this review,we will focus on the recent achievements in new materials and devices assembly,next generation therapeutics development and versatile manufacturing by combining the synthetic gene circuits,various tools and technologies from multiple fields,such as printing technology,material engineering and electronic engineering.