论文部分内容阅读
研究P选择素单克隆抗体对SCID小鼠免疫功能的影响。方法：用人胃癌组织块原位移植于SCID小鼠，制作转移模型。术后第3天开始，分别静脉注射P选择素单抗(肿瘤处理组)，每次100μg，每周2次，共3周，或PBS(肿瘤组)，每次100μl，每周2次，共3周。此外，正常组分为正常处理组与正常对照组，分别静脉注射P选择素单抗或PBS，剂量与方法同上。分别于术后第一、二、四周随机处死2只小鼠，其余均在第六周末处死。取胃癌组织、肿大淋巴结和可疑转移脏器作病理学检查；无菌取脾脏测定免疫功能。用3H-TdR掺入法测定T、B细胞转化指数，LDH释放法测定NK杀伤活性。结果：肿瘤处理组转移率低于肿瘤组。正常SCID小鼠NK活性随鼠龄增大而升高；肿瘤组第六周的免疫功能明显低于第一、二、四周，肿瘤处理组T、B细胞转化指数和NK活性在第六周时明显高于肿瘤组(均P<0.05)。结论：P选择素单抗对肿瘤转移具有抑制作用，而对小鼠细胞免疫功能无明显抑制作用。
To investigate the effect of P-selectin monoclonal antibody on immune function in SCID mice. Methods: Human gastric cancer tissue was in situ transplanted into SCID mice to make metastasis model. Beginning on day 3 after surgery, P-selectin monoclonal antibody (tumor-treated group) was administered intravenously 100 μg twice a week for 3 weeks or PBS (tumor group) 100 μl twice a week, A total of 3 weeks. In addition, the normal components of the normal control group and the normal control group, respectively, intravenous injection of P-selectin monoclonal antibody or PBS, the same dose and method. Two mice were randomly sacrificed at the first, second and fourth postoperative weeks respectively, and the rest were sacrificed on the sixth weekend. Take gastric cancer tissue, swollen lymph nodes and suspicious organs for pathological examination; aseptic spleen to measure immune function. The T, B cell transformation index was determined by 3H-TdR incorporation method, and the NK killing activity was measured by LDH release method. Results: The metastasis rate in tumor-treated group was lower than that in tumor group. The NK activity of normal SCID mice increased with age; the immune function in the sixth week of the tumor group was significantly lower than that of the first, second and fourth weeks, T, B cell transformation index and NK activity in the tumor-treated group increased Significantly higher than the tumor group (all P <0.05). Conclusion: P-selectin monoclonal antibody has inhibitory effect on tumor metastasis, but has no obvious inhibitory effect on cellular immune function.