论文部分内容阅读
目的:探讨中药人参提取物人参多糖(GP)对诱导脐血来源的单个核细胞分化为树突状细胞的形态学及免疫学机制的影响。方法:1从新鲜脐血中获得单个核细胞,接种于RPMI-1640完全培养基。对照组仅以上述培养基进行培养,实验组另加GP进行干预。2观察培养细胞超微结构,检测细胞免疫表型。3检测细胞培养液上清中GM-CSF、TNF-α、IL-10的浓度,并绘制其浓度变化图。结果:1实验组细胞呈现典型树突状细胞形态生长,可见典型DC超微结构;对照组细胞生长缓慢,无成簇生长,呈梭形巨噬细胞形态。2在细胞免疫表型学、细胞因子浓度变化的比较中,实验组与相应对照组比较均具有显著性差异。结论:1GP可诱导脐血来源的单核细胞定向分化为树突状细胞,兼具实用性和经济性,有较好的实验及临床价值。2GP可诱导单个核细胞自分泌GM-CSF、TNF-α等并促使单个核细胞向树突状细胞分化。
Objective: To investigate the effects of ginseng polysaccharides (GP) extracted from traditional Chinese medicine on the morphological and immunological mechanisms of dendritic cells derived from cord blood-derived mononuclear cells. METHODS: 1 Mononuclear cells were obtained from fresh umbilical cord blood and inoculated into RPMI-1640 complete medium. The control group was cultured only with the above medium, and the experimental group was additionally treated with GP. 2 Observe the ultrastructure of cultured cells and detect the cellular immune phenotype. 3 Detect the concentration of GM-CSF, TNF-α, and IL-10 in the supernatant of the cell culture medium, and plot the concentration change. RESULTS: 1 The cells of the experimental group showed typical dendritic cell morphology growth, and typical DC ultrastructure was seen. The cells in the control group grew slowly and did not grow in clusters. The morphology of the cells was fusiform macrophage. 2Compared with the changes of cellular immune phenotype and cytokine concentration, there was a significant difference between the experimental group and the corresponding control group. Conclusion: 1GP can induce the directional differentiation of cord blood-derived mononuclear cells into dendritic cells, which is practical and economical. It has good experimental and clinical value. 2GP can induce mononuclear cells to autocrine GM-CSF, TNF-α, etc. and promote the differentiation of mononuclear cells into dendritic cells.