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目的:探讨ALOX5AP SG13S114A/T、COX-2 765G/C和COX-1 50C/T多态性及交互作用与脑梗死的相关性。方法:采用聚合酶链反应-单核苷酸多态性方法对411例脑梗死患者(脑梗死组)和411名对照者(对照组)ALOX5AP基因SG13S114A/T、COX-2基因765G/C和COX-1基因50C/T位点多态性进行检测,应用广义多因子降维法(GMDR)检测基因与基因的交互作用。结果:ALOX5AP SG13S114 A/T、COX-1 50C/T和COX-2 765G/C基因型及等位基因分布在脑梗死组与对照组比较差异均无统计学意义;但GMDR示SG13S114和COX-2具有联合交互作用,同时携带ALOX5AP SG13S114AA型和COX-2 765CC基因型者脑梗死风险增加2.842倍。结论:对基因与基因的交互作用进行分析有助于更深入研究复杂疾病的基因型和表型间的关系。
Objective: To investigate the association between ALOX5AP SG13S114A / T, COX-2 765G / C and COX-1 50C / T polymorphisms and their interaction with cerebral infarction. Methods: The polymorphisms of ALOX5AP gene SG13S114A / T, COX-2 gene 765G / C in 411 patients with cerebral infarction (cerebral infarction group) and 411 controls (control group) were analyzed by polymerase chain reaction- single nucleotide polymorphism The 50C / T polymorphism of COX-1 gene was detected. The gene-gene interaction was detected by generalized multi-factor dimensionality reduction (GMDR). Results: The genotype and allele distribution of ALOX5AP SG13S114 A / T, COX-1 50C / T and COX-2 765G / C were not significantly different between the cerebral infarction group and the control group. However, GMDR showed that SG13S114 and COX- 2 had a combined interaction with a 2.842-fold increased risk of cerebral infarction in ALOX5AP SG13S114AA and COX-2 765CC genotypes. Conclusion: Analyzing the interaction between genes and genes may help to further investigate the relationship between genotypes and phenotypes in complex diseases.