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在 p H 1.8的 K H2 P O4 H3 P O4 缓冲介 质中,蛋白质与酸性 品红结合 使染料的 吸光度下 降。蛋白质 浓度 C 在0. 40—4.00m g · L- 1 和 8.00—40.00m g· L- 1 范 围内,吸 光度的 降低( Δ A) 与蛋白 质浓 度( C)呈 线性 关系,回 归方 程分 别为 Δ A= 0.0140 C+ 0. 0534 和 Δ A= 0. 0109 C+ 0.0895, 相关 系数 r 依次 为 0. 9965和 0.9993,方法用于奶粉和麦片中蛋白质的测定,结果满意。
In p H 1.8 K H2 P O4 H3 P O4 buffer medium, the combination of protein and acidic magenta decreases the absorbance of the dye. Protein concentration C is zero. In the range of 40-4.00 m g · L -1 and 8.00-40.00 m g · L -1, the decrease of absorbance (Δ A) was linear with the concentration of protein (C), and the regression equation was Δ A = 0.0140 C + 0. 0534 and Δ A = 0. 0109 C + 0.0895, the correlation coefficient r is 0 in turn. 9965 and 0.9993, the method for the determination of protein in milk powder and cereal with satisfactory results.