目的:探讨卵子玻璃化冷冻保存的可行性,更好提高卵子复苏率、受精率,提高卵子冻存患者的妊娠率。方法:在河北医科大学第二医院生殖医学门诊行体外受精胚胎移植(IVF-ET)患者中,由于取卵日男方取精失败行卵子冻存。于2011年2月～2012年8月共行5例患者卵子冻存并复苏。结果:冷冻MII卵子55枚,复苏率94.5%(52/55),受精率92.3%(48/52),卵裂率95.8%(46/48),移植胚胎11枚,临床妊娠3例,冷冻保存胚胎22枚。结论:卵子玻璃化冷冻是可行的,可应用于取卵日取精困难的患者。 Objective: To investigate the feasibility of vitrification of ovum and to improve the recovery rate and fertilization rate of ovum, and to improve the pregnancy rate of ovum cryopreservation patients. Methods: In IVF-ET patients in Reproductive Medicine Outpatient Department of the Second Hospital of Hebei Medical University, ovum cryopreservation was performed due to failure of sperm harvesting on the day of ovulation. Between February 2011 and August 2012, a total of 5 patients had their eggs frozen and recovered. Results: The number of cryopreserved MII eggs was 55 (94.5%, 52/55), the fertilization rate was 92.3% (48/52), the cleavage rate was 95.8% (46/48), the number of embryos transferred was 11, the clinical pregnancy rate was 3, Save 22 embryos. Conclusion: Egg vitrification is feasible and can be applied to patients who have difficulty in fetching eggs.