应用已有的猪囊尾蚴２８ＫＤａ、１８ＫＤａ、１４ＫＤａ和３４ＫＤａ抗原基因，与λｇｔ１１重组后，以液相培养法转染Ｅ．ＣｏｌｉＹ１０９０，培养表达的融合蛋白以Ｄｏｔ－ＥＬＩＳＡ法检测囊虫病人血清，其中１８ＫＤａ片段经表达后效果最好，４种片段以等比联合应用检出率最高。与粗抗原进行的ＥＬＩＳＡ、ＩＨＡ相比，具有高度的特异性及较好的敏感性，并可解决抗原来源、纯化问题 The existing 28KDa, 18KDa, 14KDa and 34KDa antigen genes of Cysticercus cellulosae were applied and recombined with λgt11 and then transfected into E. coli by liquid phase culture. ColiY1090 was used to culture the expressed fusion protein. The serum of cysticercosis patients was detected by Dot-ELISA. The 18 kDa fragment was the best, and the highest detection rate of the four fragments was obtained by the same ratio. Compared with the ELISA and IHA performed by crude antigen, it has high specificity and good sensitivity, and can solve the problem of antigen source and purification