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目的观察乙酰半胱氨酸(NAC)对四氯化碳(CCl4)诱导的原代培养大鼠肝细胞损伤的作用。方法在24孔细胞培养板上,用CCl4诱导原代培养肝细胞损伤,分别加入不同浓度的NAC,培养20 h后,测定上清液谷草转移酶(AST)活性,并MTT法检测细胞活力。结果 NAC在100μmol.L-1内,对正常培养的大鼠肝细胞没有影响;在4～100μmol.L-1内,NAC能剂量依赖性地抑制CCl4诱导的肝细胞活性的降低,EC50约为20μmol.L-1;当浓度为100μmol.L-1时,可以逆转CCl4诱导的肝损伤,使肝细胞释放AST降到接近正常水平,并明显改善肝细胞形态学变化。结论 NAC对CCl4诱导的肝损伤具有保护作用。
Objective To observe the effect of acetyl cysteine (NAC) on hepatocellular injury induced by carbon tetrachloride (CCl4) in primary cultured rat liver. Methods Primary cultured hepatocytes were induced by CCl4 on 24-well cell culture plates. Different concentrations of NAC were added. After cultured for 20 h, the activity of aspartate aminotransferase (AST) was measured and the cell viability was measured by MTT assay. Results NAC in 100μmol·L-1 had no effect on normal cultured rat hepatocytes. In the range of 4 ~ 100μmol·L-1, NAC inhibited CCl4-induced decrease of hepatocellular activity in a dose-dependent manner with EC50 of about 20 μmol·L-1. When the concentration was 100 μmol·L-1, CCl4-induced hepatic injury could be reversed, and the release of AST from hepatocytes decreased to near normal levels and markedly improved the morphological changes of hepatocytes. Conclusion NAC has a protective effect on CCl4-induced liver injury.