病毒最好从系统感染的寄主中提取,因为病毒在这些植株中的浓度比在那些受局部损伤的寄主中一般要高。用碾碎组织法或压汁法提取病毒常比用强烈的机械匀浆法为好,由于用后一种方法会使长形病毒粒体断裂。最好用冷缓冲液作为提取介质。缓冲液的类型及其 pH 值在每次提取过程中各不相 It is best to extract the virus from the host that is infected by the system because the virus is generally present in these plants at a higher concentration than those that are locally damaged. It is often better to extract the virus from ground tissue or juice than with a strong mechanical homogenization because the latter is broken by the latter method. It is best to use cold buffer as the extraction medium. The type of buffer and its pH are different for each extraction