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目的探讨转录因子T-bet和GATA-3在过敏性紫癜(HSP)患儿发病机制中的作用。方法本院儿科2009年2月-2010年2月收治急性期HSP患儿46例(HSP组)以及健康对照儿童30例(健康对照组)。采用SYBR GreenⅠ实时荧光定量PCR方法检测其外周血单个核细胞T-bet mRNA、GATA-3 mRNA的表达。应用Luminex-100液相芯片技术检测其血浆细胞因子IL-2、IL-4、IFN-γ水平。结果 HSP组患儿外周血单个核细胞T-bet mRNA相对表达水平(53.98±35.79)低于健康对照组(181.56±96.90)(P<0.001)。GATA-3 mRNA相对表达水平(964.30±655.18)高于健康对照组(78.09±57.20)(P<0.001)。HSP组血浆细胞因子IL-2[(16.54±7.38)ng.L-1],IFN-γ[(11.31±8.02)ng.L-1]的表达显著低于健康对照组[(35.73±22.66)ng.L-1,(30.67±21.29)ng.L-1](P<0.001,0.05),IL-4[(74.66±26.15)ng.L-1]的表达高于健康对照组[(51.81±27.76)ng.L-1]。IFN-γ水平与T-bet mRNA表达呈正相关(r=0.882,P<0.01);IL-4水平与GATA-3 mRNA的表达呈正相关(r=0.886,P<0.01)。结论 HSP患儿急性期存在Th1/Th2失衡,主要表现为Th2优势活化,该失衡与其特异性转录因子T-bet mRNA表达降低、GATA-3 mRNA表达增高有关,本研究为阐明Th1/Th2失衡的分子机制之一提供了实验依据。
Objective To investigate the role of transcription factors T-bet and GATA-3 in the pathogenesis of children with Henoch-Schonlein purpura (HSP). Methods 46 patients with HSP in acute stage (HSP group) and 30 healthy control children (control group) were enrolled in our hospital from February 2009 to February 2010. SYBR Green Ⅰ real-time PCR was used to detect the expression of T-bet mRNA and GATA-3 mRNA in peripheral blood mononuclear cells. The levels of IL-2, IL-4 and IFN-γ in plasma were measured by Luminex-100 liquid phase microarray. Results The relative expression of T-bet mRNA in peripheral blood mononuclear cells in HSP group was lower than that in healthy control group (53.98 ± 35.79) (181.56 ± 96.90) (P <0.001). The relative expression level of GATA-3 mRNA (964.30 ± 655.18) was higher than that of healthy controls (78.09 ± 57.20) (P <0.001). The expression of plasma cytokines IL-2 [(16.54 ± 7.38) ng.L-1 and IFN-γ [(11.31 ± 8.02) ng.L-1] in HSP group were significantly lower than those in healthy control group [(35.73 ± 22.66) (P <0.001,0.05), IL-4 [(74.66 ± 26.15) ng.L-1] was significantly higher than that in the healthy control [(51.81 ± 0.91) ng.L-1, (30.67 ± 21.29) ng.L- ± 27.76) ng.L-1]. The level of IFN-γ was positively correlated with the expression of T-bet mRNA (r = 0.882, P <0.01). The level of IL-4 was positively correlated with the expression of GATA-3 mRNA (r = 0.886, P <0.01). Conclusions There is imbalance of Th1 / Th2 in acute stage of HSP in children with Th2 predominant activation. The imbalance is related to the decrease of T-bet mRNA and GATA-3 mRNA expression. In order to clarify the imbalance of Th1 / Th2 One of the molecular mechanisms provided experimental evidence.