目的 :明确转染BMP II型突变受体对Tca8113舌癌细胞的作用 ,以进一步探讨BMPs对口腔上皮恶变的作用机制。方法 :用FuGENE6(TransfectionReagentKit)真核转染试剂盒将携带BMP II型突变受体cDNA的真核表达载体转染Tca8113细胞 (命名为Tca8113ZR细胞 ) ;然后对Tca8113ZR和Tca8113细胞分别进行生长曲线、MTT检测 ,FCM分析 ,BrdU标记检测细胞的增殖活性及DNA合成 ;以观察转染前后舌癌细胞生物学性状的变化。结果 :Tca8113ZR细胞和Tca8113细胞相比 ,形态未见明显变化 ,但是细胞的增殖活力明显下降。结论 :BMPs信号表达水平的改变在口腔上皮组织的恶变过程中起着十分重要的调控作用 OBJECTIVE: To investigate the effect of transfection of BMP II receptor on Tca8113 tongue cancer cells in order to further explore the mechanism of BMPs on oral malignant transformation. METHODS: Tca8113 cells (named as Tca8113ZR cells) were transfected with the eukaryotic expression vector carrying the BMP II mutant receptor cDNA using FuGENE6 (Transfection Reagent Kit) eukaryotic transfection kit. Then the growth curves of Tca8113ZR and Tca8113 cells were respectively analyzed by MTT Detection, FCM analysis, BrdU labeling detection of cell proliferation activity and DNA synthesis; to observe the changes of biological characteristics of tongue cancer cells before and after transfection. Results: Compared with Tca8113 cells, Tca8113ZR cells showed no obvious morphological changes, but the proliferation activity of cells decreased obviously. Conclusion: The change of BMPs expression plays an important regulatory role in the malignant transformation of oral epithelial tissues