酶法制备高等植物细胞的原生质体的研究,已有25年的历史,这为植物原生质体的培养、再生和细胞杂交等生物工程技术的基础研究和应用研究,开辟了一条广阔的道路。但是,由于高等植物的构造复杂和操作技术等各种原因,目前从原生质体再生成植株,还不广泛。仅在茄科、豆科和十字花科等十余个科计有70余种植物,获得了再生植株。 Enzymatic preparation of higher plant cell protoplasts, 25 years of history, which protoplasts of plant protoplasts, regeneration and cell hybridization and other bio-engineering technology and basic research and applied research, has opened up a vast road. However, regeneration of plants from protoplasts is not widespread at present because of the complex structure of higher plants and operating techniques. Only in Solanaceae, Leguminosae and Cruciferae and other more than 10 branches, there are more than 70 kinds of plants, access to regenerated plants.