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目的探讨Wistar大鼠重度失血性休克模型制备的影响因素。方法 70只雄性Wistar大鼠,体质量为180～220 g,麻醉后经股动脉放血40%制备重度失血性休克模型,对放血后存活大鼠动脉血中pH、PaO2、PaCO2三项指标进行正态性分析,获得其分布规律,随后计算95%参考值范围,再将所有实验大鼠按照三项指标是否均在参考值范围内分为符合组(n=61)和不符合组(n=9),通过连续校正的2检验对两组大鼠的存活与否进行分析,最后通过多重logistic回归方法分析三项指标与存活与否的相关性。结果经统计学分析发现,两组Wistar大鼠的pH值、PaO2、PaCO2值均呈偏态分布。通过计算获得存活大鼠三项指标的95%参考值范围:pH(7.30～7.59),PaO2(73～110)mmHg,PaCO2(32～50)mmHg。符合组(80.33%)与不符合组(33.33%)存活率相比差异具有统计学意义(P<0.01),其中pH值、PaCO2的交互作用与存活相关性较高。结论在重度失血性休克模型的制备中,符合组pH(7.30～7.59),PaO2(73～110)mmHg,PaCO2(32～50)mmHg范围的Wistar大鼠相对于不符合组更易于存活,制备成功率更高。其中,pH值、PaCO2的交互作用对存活与否影响更大。
Objective To investigate the influential factors of preparation of severe hemorrhagic shock model in Wistar rats. Methods Seventy male Wistar rats, weighing 180-220 g, were anesthetized and perfused with 40% bleed to prepare a model of severe hemorrhagic shock. The three indicators of arterial blood pH, PaO2 and PaCO2 in surviving rats after blood letting were positive Then the 95% reference range was calculated, and then all the experimental rats were divided into three groups according to whether the three indexes were within the reference range: n = 61 and n = 9). The survival of two groups of rats was analyzed by 校2 test of continuous correction. Finally, the correlation between the three indexes and their survival was analyzed by multiple logistic regression. Results The results of statistical analysis showed that the pH values, PaO2 and PaCO2 values of Wistar rats in both groups were skewed. The 95% reference range of the three indicators of surviving rats was obtained by calculation: pH (7.30 ~ 7.59), PaO2 (73 ~ 110) mmHg and PaCO2 (32 ~ 50) mmHg. There was significant difference (P <0.01) between survival rate of coincidence group (80.33%) and non-coincidence group (33.33%), in which pH value and PaCO2 interaction were highly correlated with survival. Conclusion In the preparation of severe hemorrhagic shock model, Wistar rats that fit the range of pH (7.30 ~ 7.59), PaO2 (73 ~ 110) mmHg and PaCO2 (32 ~ 50) mmHg were more likely to survive than the non- Higher success rate. Among them, pH, PaCO2 interaction on survival or greater impact.