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目的探讨自发性糖尿病鼠初期听力变化特点。方法清洁级雄性,7周龄Zucker Diabetes Fat(ZDF)大鼠根据糖尿病发病与否分发生糖尿病的ZDF模型鼠(fa/fa)为实验组10只,不发生糖尿病的ZDF对照鼠(fa/+)为对照组5只。分别于2、3、4、5、6月大小时测大鼠的体重、空腹血糖、短声诱发听性脑干反应(Click-ABR)、短纯音诱发听性脑干反应(tb-ABR)、畸变产物耳声发射(DPOAEs)。结果实验鼠在第2月龄内12小时禁食不禁饮测空腹血糖在正常范围,第3月龄时实验鼠的空腹血糖已达糖尿病标准并维持在较高水平;对照组大鼠血糖一直保持在正常范围。根据测得空腹血糖值大小实验组空腹血糖值上升相对较快的鼠(实验组H)在4月龄时tb-ABR在8k Hz上的阈值较其2月龄时阈值升高(P<0.05);Click-ABR 90d BSPLⅠ波潜伏期、Ⅰ-Ⅲ间期较在2月龄时延长(P<0.05),4月后Ⅰ波潜伏期、Ⅰ-Ⅲ间期开始缩短。实验组空腹血糖上升相对较慢鼠(实验组L)在5月龄时tb-ABR在8k Hz、16k Hz、32k Hz的阈值较2月龄时阈值升高(P<0.05),以32k Hz听力损失最严重,Click-ABR90d BSPLⅠ波及Ⅰ-Ⅲ波潜伏期出现较明显延长(P<0.05),5月后Ⅰ波潜伏期、Ⅰ-Ⅲ间期开始缩短。对照组大鼠实验前后听力无变化(P>0.05)。结论肥胖ZDF糖尿病大鼠发病前期听力无明显变化,糖尿病鼠发病稳定后1到2个月(即4、5月龄)开始出现较明显的听力损害;糖尿病听力损失的发病进展速度也许与血糖升高的快慢有关;糖尿病鼠高频听力损失较重,提示临床糖尿病人应当监测高频听力变化。
Objective To investigate the characteristics of initial hearing changes in spontaneous diabetic rats. Methods 10 male ZDFer rats (ZDF rats) with diabetes at 7 weeks of age were divided into experimental group (n = 10) and diabetic ZDF control rats (fa / ) For the control group of 5. Body weight, fasting blood glucose, Click-ABR and short timbre-induced auditory brainstem response (tb-ABR) were measured at 2, 3, 4, , Distortion product otoacoustic emissions (DPOAEs). Results In the first 2 months of age, the fasting 12-hour fasting test can not help but test the fasting blood glucose in the normal range. At 3 months of age, the fasting blood glucose of the experimental rats reached the standard of diabetes and maintained at a high level. The blood glucose of the control rats remained In the normal range. The threshold of tb-ABR at 8k Hz at 4 months was higher than that at 2 months of age (P <0.05) according to the fasting blood glucose level measured in the experimental group (P <0.05) ). The latency of BSPLⅠ wave at 90-day Click-ABR was prolonged (P <0.05). The I-wave latency and Ⅰ-Ⅲ interval began to shorten after April. In the experimental group, the threshold of tb-ABR at 8k Hz, 16k Hz and 32k Hz was higher than that at 2-month-old (P <0.05) The hearing loss was the most serious. The latencies of wave Ⅰ-Ⅲ in Click-ABR90d group were significantly prolonged (P <0.05). The latencies of Ⅰ wave and Ⅰ-Ⅲ interval began to shorten after May. The control group had no change in hearing before and after the experiment (P> 0.05). Conclusion Obese ZDF diabetic rats did not show any significant changes in hearing during the early stage of the disease. Diabetic rats started to show obvious hearing impairment within 1 to 2 months (ie, 4 to 5 months) after onset of stable disease. The incidence of diabetic hearing loss may be related to the increase of blood glucose High speed related; high frequency hearing loss in diabetic mice heavier, suggesting that clinical diabetic patients should monitor changes in high-frequency hearing.