小鼠肥胖不同阶段相关免疫器官恒定自然杀伤T细胞(iNKT)的频率和亚群变化

来源 :中华微生物学和免疫学杂志 | 被引量 : 0次 | 上传用户:loverbeyond
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:采用高脂饮食喂养构建小鼠肥胖模型,观察恒定自然杀伤T细胞(iNKT)在肥胖不同阶段脂肪及相关组织中的频率及亚群变化,探讨iNKT细胞在肥胖脂肪组织慢性炎症中的作用。方法:记录小鼠体重和精神状态以及葡萄糖耐量和胰岛素耐量的变化;苏木精-伊红(HE)染色观察脂肪组织病理变化;流式细胞术检测iNKT细胞和巨噬细胞频率和亚群的变化;CBA检测血清和脂肪组织淋巴细胞培养上清中细胞因子水平;Western blot检测脂肪组织中相关蛋白质的表达量差异。结果:(1)高脂喂养4周后,脂肪细胞体积明显增大,炎性细胞浸润不明显;高脂喂养12周后,炎细胞浸润明显增多。(2)高脂喂养可致脂肪组织中iNKT细胞频率下降,iNKT1亚群比例增高,iNKT10亚群比例下降;胸腺iNKT1亚群比例升高,iNKT2亚群比例下降;脂肪组织中巨噬细胞频率及M1亚群比例增高,M2亚群比例下降,上述变化在高脂喂养12周组更为明显。(3)高脂喂养造成脂肪组织中E4BP4、精氨酸酶1(Arg-1)表达下降,诱导型一氧化氮合酶(iNOS)表达增高。(4)高脂喂养使小鼠血清和脂肪组织淋巴细胞培养上清中促炎性细胞因子明显增高,抑炎性细胞因子显著下降,且12周组变化更为明显。结论:肥胖小鼠脂肪组织中iNKT1细胞亚群升高和iNKT10细胞亚群降低与M1型极化的增强可能存在密切关系,iNKT细胞亚群的失衡可能参与了肥胖小鼠脂肪组织慢性炎症的进展。“,”Objective:To observe the changes in percentages and subsets of invariant nature kiler T (iNKT) cells in adipose and related tissues at different stages of obesity, and analyze the role of iNKT cells during chronic inflammation in adipose tissues in a mouse model of obesity established with high-fat diet.Methods:Changes in mouse body weight, mental state, glucose tolerance and insulin tolerance were recorded. Hematoxylin and eosin (HE) staining was used to observe pathological changes in adipose tissues. Flow cytometry was performed to detect the percentages and subsets of iNKT cells as well as the percentages and subtypes of macrophages. The levels of cytokines in serum samples and the culture supernatants of lymphocytes in adipose tissues were detected with CBA. The expression of related proteins in adipose tissues was detected by Western blot.Results:(1) The volume of adipose cells increased significantly after four weeks of high-fat feeding, but the infiltration of inflammatory cells was not obvious. Significantly increased infiltration of inflammatory cells was observed after 12 weeks of high-fat feeding. (2) High-fat feeding could reduce the percentage of iNKT cells, increase the proportion of iNKT1 subgroup and decrease the proportion of iNKT10 subgroup in adipose tissues. The proportion of iNKT1 subgroup in thymus increased, but that of iNKT2 subgroup decreased. The percentage of macrophages and the proportion of M1 subgroup in adipose tissues increased, while the proportion of M2 subgroup decreased, which were more obvious after 12 weeks of high-fat feeding. (3) High-fat feeding resulted in decreased expression of E4BP4 and arginase-1 (Arg-1) in adipose tissues and increased expression of inducible nitric oxide synthase (iNOS). (4) High-fat feeding significantly increased the pro-inflammatory cytokines and decreased the anti-inflammatory cytokines in mouse serum and culture supernatants of lymphocytes in adipose tissues with more significant changes observed after 12 weeks of high-fat feeding.Conclusions:Increased iNKT1 and decreased iNKT10 in obese adipose tissues might be closely related to the increased M1 polarization and the imbalance of iNKT subsets might be involved in the progression of chronic inflammation in obese adipose tissues.
其他文献
会议
一种用于公路桥梁收费道口或隘口处以拦截逃票和问道车辆的KD2型快速挡板,于1995年9月中旬在合肥哈福机电技术研究所研制成功。 该产品主要用于公路收费道口处,拦截闯道逃费
不知不觉,牛年已悄然而至.说起牛,你知道它“牛”在哪吗?我们身边的大部分家养动物大多各自起源于同一个物种,例如:世界各地的家马、东西方的家猪以及形态迥异的家犬.然而,家
期刊
卜_、._,、___。二.“——--。。’.、、X回回回h_-缀圈 巴 二W 主 丫。_---_-._-一、_.丫撅二回回回回回回回回回*缀镶缀-_ 山 缀缀、二二— 一,”、 摄一缀缀 一、入。 人
社会经济的高速发展,我国水利泵站的建设规模也逐渐扩大,对于泵站电气设备的维护管理工作也逐渐受到了广泛专家学者的关注.基于此,下文将对水利泵站之中的电气设备运行维护与
基木技术指标(项目)数字电视技术指标(参数要求)数字信号输人格式全面兼容现有HI)1’v三种模式一25()像素(水平)x72o像素(垂直)P/6oHz(逐行扫描)一欧美NT5c制192()像素{水平)
提到牛,人们熟悉的有黑白花的大奶牛、勤劳耕作的老黄牛、毛皮黑漆漆的水牛……为什么有的牛是单一颜色的,而有的牛是黑白花纹的呢?在自然界中,除了牛,还有很多动物身上有斑
期刊
同型半胱氨酸与疾病的关系同型半胱氨酸(Hcy)是蛋氨酸代谢产物,在叶酸循环和蛋氨酸循环中起重要作用de Jager DJ,et al.JAMA 2009;302:1782Hcy血症被认为是心血管疾病的非传
会议
常言道:患难见真情。杨勇和警卫员 孙启增的友谊,正是在1967年1月,杨 勇一家遭受劫难时得到真实的表现。 在京西宾馆的交锋 1967年1月12日,新的全军文革小组成立,组长徐向前
当彩电出现无光、无声故障时,要判定故障是在开关电源还是在行扫描电路,这样才能进一步检修。而现在的一些机型往往保护电路较多,行扫描电路出现故障时,导致开关电源无输出,