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目的：探讨血管紧张素Ⅱ（Ａｎｇ Ⅱ）在肾间质纤维化过程中的作用机制。 方法：应用ＭＴＴ 比色法检测Ａｎｇ Ⅱ对成纤维细胞的增生，ＲＴＰＣＲ 检测纤溶酶原激活物抑制物（ＰＡＩ１）ｍＲＮＡ 的表达。 结果：Ａｎｇ Ⅱ可促进肾间质成纤维细胞增生及ＰＡＩ１ ｍＲＮＡ 的表达，且氯沙坦（１０ －４ｇ／Ｌ） 可抑制Ａｎｇ Ⅱ的促增生作用。 结论：Ａｎｇ Ⅱ可能通过直接作用于肾间质成纤维细胞，促进其增生，同时抑制细胞外基质的降解而参与肾间质纤维化的过程，而Ａｎｇ Ⅱ受体拮抗剂拮抗Ａｎｇ Ⅱ促细胞增生作用。
Objective: To investigate the mechanism of angiotensin Ⅱ (Ang Ⅱ) in renal interstitial fibrosis. Methods: The proliferation of fibroblasts was detected by MTT colorimetric assay, and the expression of plasminogen activator inhibitor (PAI-1) mRNA was detected by RT-PCR. RESULTS: Ang Ⅱ promoted the proliferation of renal interstitial fibroblasts and the expression of PAI-1 mRNA. Losartan (10 -4 g / L) could inhibit the growth-promoting effect of Ang Ⅱ. CONCLUSION: Ang Ⅱ may participate in the process of renal interstitial fibrosis by directly acting on interstitial fibroblasts, promoting its proliferation and inhibiting the degradation of extracellular matrix. However, Ang Ⅱ antagonist antagonizes Ang Ⅱ promoting cell proliferation effect.