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1 器材与试剂准备 白铁皮制长条形内分4～5格脱水盒,宽度以能放进大口磨石玻瓶为度;500ml大口磨砂带盖玻瓶6只,盛脱水剂用。硬脂酸(AR)。其余试剂器材同日常切片用。2 制片程序2.1 6～8h脱水浸蜡包埋法:①下午下班前取材,厚度1mm,放脱水盒内盖好,放入盛AF的大口玻瓶内过夜。②翌日取出,依次放入:第一瓶无水乙醇内60min—→二瓶无水乙醇40min—→三瓶无水乙醇40min—→丙酮内40min,取出,将标本换入浸蜡盒内,保持65～67℃浸蜡3～5h,包埋(浸包蜡配方:切片石蜡10份加硬脂酸1份,如用工业石蜡,再加蜂蜡1份)。
1 Equipment and reagents Preparation White metal strip long 4 to 5 boxes within the dehydration box, the width to be able to put a large grindstone glass bottle for the degree; 500ml large mouth matte cover glass bottle 6, Sheng dehydrator. Stearic acid (AR). The remaining reagent equipment used with the daily slice. 2 Preparation procedures 2.1 6 ~ 8h dehydration wax embedding method: ① Afternoon take the material before get off work, the thickness of 1mm, put the dehydration box lid, put Sheng FENG large mouth glass bottle overnight. ② the next day to remove, turn into: the first bottle of anhydrous ethanol 60min- → two bottles of ethanol 40min- → three bottles of anhydrous ethanol 40min- → acetone 40min, remove the specimen into the wax box to maintain 65 ~ 67 ℃ immersion wax 3 ~ 5h, embedded (immersion wax formulations: slice paraffin 10 parts stearic acid 1, such as industrial paraffin, together with beeswax 1).