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采用聚丙烯酰胺凝胶电泳(PAGE)对洞穴鱼类——湘西盲高原鳅(Triplophysa xiangxiensis)群体遗传多样性进行了研究。对其肌肉、心脏、肝脏、脑、脾脏和肾脏等6种组织中的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、酯酶(EST)、醇脱氢酶(ADH)、谷氨酸脱氢酶(GDH)和葡萄糖-6-磷酸脱氢酶(G-6-PDH)等7种同工酶进行分析,检测到26个座位,其中多态座位4个(Ldh-3、Ldh-4、s-Mdh、m-Mdh),多态座位比例为15.38%,观察杂合度0.0398,预期杂合度0.0411,Hardy-Weinberg遗传偏离指数(D)为-0.0316,存在杂合子缺失。结果表明,湘西盲高原鳅群体群体遗传多样性较低,纯合度较高。
Polyacrylamide gel electrophoresis (PAGE) was used to study the genetic diversity of cavefish Triplophysa xiangxiensis. Lactate dehydrogenase (LDH), malate dehydrogenase (MDH), malic enzyme (ME), esterase (EST), and alcohol in 6 tissues including muscle, heart, liver, brain, spleen and kidney ADH, GDH and G-6-PDH, we detected 26 loci, of which polymorphism The four loci (Ldh-3, Ldh-4, s-Mdh and m-Mdh) had 15.38% polymorphic loci, the observed heterozygosity was 0.0398, the expected heterozygosity was 0.0411 and the Hardy-Weinberg genetic divergence index was - 0.0316, there is a loss of heterozygotes. The results showed that the genetic diversity of Populus euchinensis population was low and the homozygosity was high.