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AIM: To verify the expression and methylation status o the MAGE-A1 and MAGE-A3 genes in colorectal cance tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as we as sequencing analysis, after sodium bisulfite modifica tion in 32 colorectal cancer cell lines and 87 cancer tis sues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respective ly. Subsequent to sodium bisulfite modification and MS PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77% respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcino mas as well as in gastric cancers and might play a signif icant role in the development and progression of human colorectal carcinomas.
AIM: To verify the expression and methylation status o the MAGE-A1 and MAGE-A3 genes in colorectal cance tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as we as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tis sues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE- were found in 59% and 66%, respectively ly. Subsequent to sodium bisulfite modification and MS PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both 81% each Promoter hypomethylation of MAGE-A1 and MAGE- A3 in colorectal cancer tissues was observed in 43% and 77% respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6% respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up- regulates its expression in colorectal c arcino mas as well as in gastric cancers and might play a signif icant role in the development and progression of human colorectal carcinomas.