Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flowering. We previously reported that there are at least six FT genes(GmFTLl-6) in soybean displaying flowering activity. However, the individual functions of genes GmFTLl-6 remain to be identified. In this study, we cloned the GmFTL2 promoter(GmFTLpro) from soybean(Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR(qRT-PCR). In GmFTLpro::GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and the development of micro game tophytes, embryos, and roots. Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T (FT) gene is well-known florigen, which integrates various signals from multiple flowering-regulation pathways to flow flow. However, the individual functions of genes GmFTL1-6 remain to be identified. In this study, we cloned the GmFTL2 promoter (GmFTLpro) from soybean (Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR (qRT-PCR). In GmFTLpro :: GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and th e development of micro game tophytes, embryos, and roots.