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目的:通过检测客家人群冠心病患者HO-1基因启动子区GT重复多态性,旨在阐明HO-1基因多态性与冠心病易感性的关联。方法:选择广东梅州客家地区确诊的冠心病患者100例为病例组,经冠状动脉造影或无创检查排除冠心病的个体100例为对照组。采用荧光标记PCR和毛细管电泳相结合技术检测HO-1启动子区(GT)n多态性并进行基因分型,结合冠心病危险因素,比较携带不同等位基因/基因型的患者冠心病的患病风险。结果:(GT)n重复次数在16~39之间,将(GT)n重复系列分为L(n>30)和S(n≤29)2种等位基因,S/S、S/L、L/L3种基因型。病例组与对照组个体基因型分布差异无统计学意义(校正后OR=1.13,95%CI=0.95~1.24),但分层分析后发现携带L等位基因(S/L+L/L基因型)的糖尿病个体冠心病的发病风险增加更显著(校正后OR=2.29,95%CI=1.06~4.80)。结论:HO-1基因多态性与客家人群冠心病易感性相关,能明显增加糖尿病个体冠心病患病风险。
OBJECTIVE: To detect the association of HO-1 gene polymorphism with the susceptibility to coronary heart disease (CHD) by detecting GT repeat polymorphisms in the HO-1 gene promoter region of patients with coronary heart disease in the Hakka population. Methods: One hundred patients with coronary heart disease diagnosed in Hakka, Meizhou, Guangdong Province were selected as the case group. One hundred patients who had coronary artery disease excluded by coronary angiography or noninvasive examination were selected as the control group. The polymorphisms of HO-1 promoter (GT) n were detected by fluorescence-labeled PCR and capillary electrophoresis combined with genotyping. The risk factors of coronary heart disease were compared with those of patients with different alleles / genotypes Risk of illness. Results: The number of (GT) n repeats was between 16 and 39, and the (GT) n repeat series were divided into two kinds of alleles of L (n> 30) and S (n≤29) , L / L3 genotypes. There was no significant difference in genotype distribution between case group and control group (corrected OR = 1.13, 95% CI = 0.95-1.24). However, stratified analysis showed that allele L (S / L + L / L) Type) diabetes patients with coronary heart disease risk increased significantly more significant (adjusted OR = 2.29, 95% CI = 1.06 ~ 4.80). Conclusion: HO-1 gene polymorphism is associated with susceptibility to coronary heart disease in the Hakka population, which can significantly increase the risk of coronary heart disease in diabetic individuals.