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目的：探讨Ｋ－ｒａｓ，ｐ５３基因表达与人胰腺癌临床生物学行为之间的关系。方法：采用免疫组织化学与斑点杂交方法，检测４８例人胰腺癌组织Ｋ－ｒａｓ，ｐ５３基因表达。结果：胰腺癌组织Ｐ２１ｒａｓ蛋白表达较正常胰腺组织增强（Ｐ＜０．０５），Ⅰ～Ⅱ期较Ⅲ～Ⅳ期表达增强（Ｐ＜０．０５），组织学分级Ｇ１，Ｇ２级较Ｇ３级表达增强（Ｐ＜０．０１），Ｐ２１ｒａｓ蛋白表达阳性患者生存期较表达阴性者延长（Ｐ＜０．０１）；胰腺癌组织Ｐ２１ｒａｓ蛋白与Ｋ－ｒａｓｍＲＮＡ表达呈正相关（ｒ＝０．８９，Ｐ＜０．０１）；胰腺癌组织Ｐ５３蛋白表达较正常胰腺及慢性胰腺炎组织增强（Ｐ＜０．０５），Ⅲ～Ⅳ期胰腺癌较Ⅰ～Ⅱ期Ｐ５３蛋白表达增强（Ｐ＜０．０５），随肿瘤分化程度降低Ｐ５３蛋白表达而增强（Ｐ＜０．０１），Ｐ５３蛋白与ｐ５３ｍＲＮＡ表达呈负相关（ｒ＝－０．４４，Ｐ＜０．０１）。结论：Ｐ２１ｒａｓ与Ｐ５３蛋白表达对估价胰腺癌患者的预后具重要价值；Ｋ－ｒａｓｍＲＮＡ高表达是Ｐ２１ｒａｓ蛋白表达增强的重要机制之一
Objective: To investigate the relationship between K-ras, p53 gene expression and the clinical biological behavior of human pancreatic cancer. Methods: The expression of K-ras and p53 genes in 48 pancreatic cancer tissues were detected by immunohistochemistry and dot blot hybridization. RESULTS: The expression of P21ras protein in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues (P<0.05). The expression of P21ras protein in stage I-II was higher than that in stage III-IV (P<0.05). The histological grades G1, G2 were higher than G3. Increased expression (P<0.01). The survival of patients with positive expression of P21ras protein was longer than that of patients with negative expression (P<0.01). There was a positive correlation between P21ras protein and K-ras mRNA expression in pancreatic cancer tissues (r=0.89, P). <0.01); The expression of P53 protein in pancreatic cancer tissue was significantly higher than that in normal pancreas and chronic pancreatitis (P<0.05). The expression of P53 protein in stage III-IV pancreatic cancer was higher than that in stage I-II (P<0.05). ), P53 protein expression increased with the decrease of tumor differentiation (P<0.01), P53 protein was negatively correlated with p53 mRNA expression (r=-0.44, P<0.01). Conclusion: The expression of P21ras and P53 protein is of great value in evaluating the prognosis of patients with pancreatic cancer; the high expression of K-ras mRNA is one of the important mechanisms of the enhanced expression of P21ras protein.