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目的 观察斜视性弱视猫治疗前后视皮质 (VisralCortex ,VC) 17区神经生长因子(NGF)表达改变 ,探讨神经生长因子与视觉发育可塑性的联系。方法 普通 4周龄家猫 18只 ,随机分为正常对照组、斜视组和斜视治疗组 ,每组各 6只 ,后两组 4周龄时行右眼外直肌切断术 ,术后 4周经P -VEP检测确定形成弱视后立即将治疗组 6只猫行对侧眼睑暂时缝合。三组猫全部于 12周龄处死 ,取右脑半球VC17区脑组织 ,应用免疫组织化学染色法观察 17区NGF免疫阳性细胞密度。结果 斜视组与正常组相比 :免疫阳性细胞数量明显减少 (P <0 0 5 ) ,P -VEP的P1波潜时延迟 ,波幅降低 ;治疗组和斜视组相比 ,免疫阳性细胞增加 (P <0 0 5 ) ,P1波潜时缩短 ,波幅增大。结论 斜视性弱视猫VC17区NGF表达特性发生改变 ,通过遮盖对侧眼 ,增加弱视眼的视觉信息输入量 ,NGF表达增加 ,和P -VEP的改变相符合 ,遮盖治疗使实验猫弱视眼的视功能得到一定的改善。NGF作为一种细胞功能的调控因子 ,在视觉系统发育的可塑性方面发挥重要作用。
Objective To observe the changes of nerve growth factor (NGF) in the 17th area of the Visal Cortex (VC) before and after treatment of strabismic amblyoptic cats, and to explore the relationship between the nerve growth factor and the visual developmental plasticity. Methods 18 normal 4-week-old domestic cats were randomly divided into normal control group, strabismus group and strabismus treatment group, 6 in each group. The latter two groups were performed right lateral rectus ablation at 4 weeks of age. After 4 weeks The P -VEP test to determine the formation of amblyopia immediately after the treatment group 6 cats temporary contralateral eyelid suture. All three cats were sacrificed at 12 weeks of age, and the brain tissue of VC17 in the right hemisphere was taken. Immunohistochemical staining was used to observe the density of NGF immunoreactive cells in zone 17. Results Compared with the normal group, the numbers of immunopositive cells in the strabismic group were significantly decreased (P <0.05). The latency of the P1 wave in the P-VEP group was delayed and the amplitude was decreased. Compared with the strabismus group, the number of immunopositive cells increased <0 0 5), P1 wave latency is shortened, the amplitude increases. Conclusion The expression of NGF in VC17 area of strabismic amblyopia cats is changed. By covering the contralateral eye and increasing the visual information input of amblyopic eyes, the expression of NGF is increased, which is consistent with the change of P-VEP. Function to be some improvement. NGF, as a regulator of cellular function, plays an important role in the plasticity of visual system development.