在大棚水泥池内种植两个Bt玉米(5422Bt1和5422CBCL)及其同源常规玉米5422,研究了种植Bt玉米及秸秆还田过程中根际土、根围土、3层根外土(0~20、20~40和40~60cm)中Bt蛋白含量的时空动态特征及其对土壤养分含量的影响.结果表明:种植Bt玉米和常规玉米后,根围土(种植后90 d)和3层根外土(种植后30、60和90 d)中均检测到少量的Bt蛋白(含量<0.5 ng·g-1),在Bt玉米5422Bt1和5422CBCL根际土中则分别检测到1.59和2.78 ng·g-1的Bt蛋白.玉米秸秆还田后,Bt蛋白能在3 d内快速降解,在还田后第7天只检测到少量的Bt蛋白.与常规玉米5422相比,种植Bt玉米5422Bt1 90 d后根围土和3层根外土中有机质、速效养分(碱解氮、速效磷和速效钾)和全量养分(全氮、全磷和全钾)含量均没有显著差异;5422Bt1秸秆还田60 d后0~20 cm土层的有机质和全氮含量显著升高,速效钾含量显著降低,而其他养分指标则没有显著差异,20~40 cm和40~60 cm土层的所有养分指标均没有明显差异.种植Bt玉米5422CBCL后根围土中仅速效磷含量显著低于种植常规玉米5422,但0~20 cm土层中全磷含量显著提高,其他养分指标均没有差异;还田5422CBCL秸秆后仅0~20 cm土层的速效磷含量显著高于常规玉米5422.研究结果表明,通过玉米根系分泌和秸秆分解进入土壤的Bt蛋白不会在土壤中累积,对养分含量也基本没有显著影响. Two Bt corn (5422Bt1 and 5422CBCL) and their homologous conventional corn 5422 were planted in a greenhouse of greenhouses. Rhizosphere soil, rhizosphere soil, three-layer root and outer soil (0-20, 20 ~ 40 and 40 ~ 60cm), and their effects on soil nutrient contents were studied.The results showed that after Bt corn and conventional maize were planted, the effects of Bt corn (90 days after planting) A small amount of Bt protein (content <0.5 ng · g-1) was detected in soil (30, 60 and 90 d after planting), and 1.59 and 2.78 ng · g in Bt corn 5422Bt1 and 5422CBCL rhizosphere respectively -1 Bt protein.Bt returned to the field after straw, Bt protein rapidly degraded within 3 d, only a small amount of Bt protein was detected on the 7th day after returning to the field.Compared with the conventional corn 5422, Bt corn 5422Bt1 90 d There was no significant difference in the content of available organic matter, available nutrients (available nitrogen, available phosphorus and available potassium) and total nutrients (total nitrogen, total phosphorus and total potassium) The content of organic matter and total nitrogen in 0 ~ 20 cm soil layer increased significantly after d, while the content of available potassium decreased significantly, but no significant difference was observed in other nutrients, 20 ~ 40 cm And 40 ~ 60 cm soil layer, there was no significant difference in all nutrient indexes.After application of Bt corn 5422CBCL, the content of available P only was significantly lower than that of conventional corn 5422, but the content of total phosphorus in 0-20 cm soil layer was significantly increased , And no significant difference was found in other nutrients.After returning 5422CBCL straw, available phosphorus content in 0-20 cm soil layer was significantly higher than that of conventional corn 5422. The results showed that Bt protein entering the soil through corn root excretion and straw decomposition was not Soil accumulation, the nutrient content of the basic no significant effect.