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植物真菌病害给农业生产带来了巨大损失,因此对高效、低毒、低残留的生物农药的开发迫在眉睫。洋葱伯克霍尔德菌CF-66(Burkholderia cepacia CF-66)对真菌类病原菌具有强烈的抑制作用。其发酵液经减压浓缩和乙酸乙酯萃取得到粗提液,粗提液经反复硅胶柱层析和反相高效液相色谱(RP-HPLC)多步柱层析,首次分离纯化得到一种环二肽——cyclo(Phe-Pro)(cFP)。利用气质联用(GC-MS)系统和HPLC进行定性和定量,结果表明分离纯化物质呈单峰,纯度较高且经标准曲线算出其浓度约为15 mg/ml。MIC值的测定结果表明该物质对立枯丝核菌、黄瓜菌核、玉米弯孢病菌等植物病原菌及冻土毛霉、黄曲霉、米根霉等食品腐败菌均具有较强的抑制作用。经显微镜观察发现,该物质可使丝状真菌菌丝生长异常,菌丝由光滑细长变得粗糙、弯曲、短粗且顶端膨大呈泡状。
Plant fungal diseases have brought huge losses to agricultural production, so the development of efficient, low toxicity and low residual bio-pesticides is imminent. Burkholderia cepacia CF-66 has a strong inhibitory effect on fungal pathogens. The fermentation broth was concentrated under reduced pressure and extracted with ethyl acetate to obtain a crude extract. The crude extract was purified by repeated column chromatography on silica gel and reversed-phase high performance liquid chromatography (RP-HPLC) Cyclic dipeptide -cyclo (Phe-Pro) (cFP). Qualitative and quantitative analysis by GC-MS and HPLC showed that the isolated and purified material showed a single peak with high purity and a concentration of about 15 mg / ml as calculated by standard curve. The results of MIC value showed that this material had a strong inhibitory effect on plant pathogens such as Rhizoctonia solani, Cucumber sclerotiorum, Curvularia lunata and other spoilage organisms such as Mucor, Frozen Rhizopus, Rhizopus oryzae. Microscopically, it was found that this material caused the mycelial growth of filamentous fungi to be abnormal. The mycelium became smooth, slender and long, curved, short and thick, and its tip swollen and bubbled.