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[目的]探讨苓桂术甘汤对非酒精性脂肪性肝炎(NASH)大鼠肝组织DGAT2、PKCε的影响。[方法]高脂饮食饲养SD大鼠8周制备NASH模型,药物治疗4周后,自动生化分析仪检测血清肝功能(AST、ALT)、血脂(CHO、LDL、HDL、TG)水平,苏木精-伊红染色、油红O染色观察肝组织病理,RT-PCR检测DGAT2mRNA、PKCεmRNA,Western-blot检测DGAT2和PKCε蛋白的表达。[结果]苓桂术甘汤组、罗格列酮组能明显改善肝组织的脂肪变性和炎症程度,降低血清TG、ALT、AST,下调DGAT2、PKCεmRNA和蛋白的表达水平(P<0.05、P<0.01)。[结论]苓桂术甘汤可能是通过下调肝组织DGAT2、PKCεmRNA和蛋白的表达水平,对肝内脂质代谢和胰岛素抵抗起到了调节和改善作用,从而达到治疗NASH的目的,DGAT2/PKCε有可能成为治疗NASH的新靶点。
[Objective] To explore the effect of Lingguizhugan decoction on the hepatic tissue DGAT2 and PKCε in nonalcoholic steatohepatitis (NASH) rats. [Methods] The NASH model was induced in SD rats for 8 weeks by high-fat diet. After 4 weeks of drug treatment, the levels of serum hepatic function (AST, ALT), serum lipids (CHO, LDL, HDL, TG) Eosin staining and oil red O staining were used to observe the pathological changes of liver tissues. DGAT2 mRNA and PKCε mRNA were detected by RT-PCR, and the expression of DGAT2 and PKCε protein were detected by Western-blot. [Results] Lingguizhugan decoction and rosiglitazone group could significantly improve the degree of steatosis and inflammation in liver tissue, decrease serum TG, ALT, AST, and down-regulate the expression of DGAT2, PKCεmRNA and protein (P <0.05, P < 0.01). [Conclusion] Lingguizhugan Decoction may regulate and improve the intrahepatic lipid metabolism and insulin resistance by down-regulating the expression of DGAT2, PKCεmRNA and protein in liver tissue so as to achieve the goal of treating NASH. DGAT2 / PKCε may be Become a new target for the treatment of NASH.