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目的:为了观察通腑泻热灌肠合剂对大鼠腹腔粘连组织的影响,探讨预防术后肠粘连的机理。方法:将大鼠肠粘连模型的腹腔粘连组织进行LSAB法染色,光镜观察IgG抗原的表达率、表达水平及在组织细胞的分布。结果:中药组的抗原表达率与盐水组和对照组相比抗原表达率显著降低(P<0.01),阳性细胞数少于20%,同时在组织细胞上表现出少量胞膜阳性,阳性细胞反应散在。结论:IgG抗原的表达与炎症反应程度有关,腹腔浆膜损伤伴炎症反应的发生,显示在一定程度上,通腑泻热灌肠合剂能降低组织炎症反应,对IgG抗原的表达有一定的影响。
OBJECTIVE: To observe the effect of Tongqiao Xiexue Enema Mixture on rat abdominal adhesion tissue and to explore the mechanism of preventing postoperative intestinal adhesion. METHODS: The peritoneal adhesions of rat intestinal adhesion model were stained with LSAB. The expression rate of IgG antigen, expression level and distribution in tissue cells were observed by light microscope. RESULTS: The antigen expression rate of the Chinese medicine group was significantly lower than that of the saline group and the control group (P<0.01), and the number of positive cells was less than 20%. At the same time, a small amount of positive cells were found on the tissue cells. The cellular reaction is scattered. Conclusion: The expression of IgG antigen is related to the degree of inflammatory reaction. The occurrence of inflammatory reaction in the abdominal cavity with serous membrane injury shows that Tongfu Xiexige enterotherapy mixture can reduce the inflammatory response of tissues and has an impact on the expression of IgG antigen.