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胎儿与损伤DNA的物质接触能引起异常分娩、癌变和畸形。作者提出一种新方法,通过老鼠的SCE检查其胎儿DNA的损伤。于妊娠后第11、15、19天静脉注射BrdU,24小时后杀死动物,获得母体和胎儿组织的中期分裂相细胞,同时观察胎儿细胞和母体骨髓细胞的SCE频率。胎儿细胞的基础SCE频率低于母体,前者的第三复制周期细胞的SCE为2.4～3.6,并不随胎龄而改变。用三种已知的致突变药物(环磷酰胺,
Fetal contact with damaged DNA can cause abnormal delivery, cancer and deformity. The authors propose a new approach to examine fetal DNA damage through SCE in mice. BrdU was intravenously injected on days 11, 15, and 19 after gestation, and animals were sacrificed 24 hours later to obtain metaphase cells of maternal and fetal tissues. The frequencies of SCE in fetal cells and maternal bone marrow cells were also observed. The basal SCE frequency of fetal cells was lower than that of maternal cells, and the SCE of the former cells in the third replication cycle was 2.4-3.6, which did not change with gestational age. Three known mutagenic drugs (cyclophosphamide,