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直接注射DNA是一种有效的疫苗接种方法,可同时诱导体液和细胞介导免疫应答,可制成结合疫苗;更重要的是,由于载体本身不诱导免疫应答,因此,同样的或类似的载体可用于嗣后免疫接种。用作DNA疫苗的载体必须具备以下特点:在哺乳动物细胞内,能高水平表达病毒基因,但不能复制,不会整合到宿主染色体中。 据此,作者构建了以V1与PUC19为基础的V1J载体系列。它们以去除lac操纵子的PUC19为骨架,将巨细胞病毒（CMV）IE1增强子、启动子和内含子A转录调节因子与其连接,同时,用BGH poly（A）序列启动目的基因氯霉素乙酰转移酶（CAT）或甲型流感病毒核蛋白（NP）或血凝素（HA）表达。为了检测V1与V1J质粒中CAT基因的表达,作者将构建好的质粒转入小鼠成肌细胞系（G8）、人成横纹肌细胞瘤细胞系（RD）与猴肾细胞系（CV-1）中,并通过放射自显影实验进行比较,发现V1J-CAT在三种细胞系中
Direct injection of DNA is an effective method of vaccination that induces humoral and cell-mediated immune responses at the same time to make a conjugate vaccine; more importantly, since the vector itself does not induce an immune response, the same or similar vectors Can be used for subsequent immunization. Vectors used as DNA vaccines must have the following characteristics: In mammalian cells, the virus gene can be expressed at high levels, but can not be copied and not integrated into host chromosomes. As a result, the authors have developed a family of V1J vectors based on V1 and PUC19. They linked cytomegalovirus (CMV) IE1 enhancer, promoter and intron A transcription regulators to PUC19, which removes the lac operon, and chloramphenicol Acetyltransferase (CAT) or influenza A virus nucleoprotein (NP) or hemagglutinin (HA). In order to detect the expression of CAT gene in V1 and V1J plasmids, the constructed plasmids were transfected into mouse myoblast cell line (G8), human stromal cell line RD and monkey kidney cell line CV-1, , And compared by autoradiography experiments, it was found that V1J-CAT was found in three cell lines